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KMID : 0606920140220020136
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Biomolecules & Therapeutics
2014 Volume.22 No. 2 p.136 ~ p.142
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The Polyphenol Chlorogenic Acid Attenuates UVB-mediated Oxidative Stress in Human HaCaT Keratinocytes
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Cha Ji-Won
Piao Mei Jing
Kim Ki-Cheon
Yao Cheng Wen
Zheng Jian
Kim Seong-Min
Hyun Chang-Lim
Ahn Yong-Seok
Hyun Jin-Won
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Abstract
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We investigated the protective effects of chlorogenic acid (CGA), a polyphenol compound, on oxidative damage induced by UVB exposure on human HaCaT cells. In a cell-free system, CGA scavenged 1,1-diphenyl-2-picrylhydrazyl radicals, superoxide anions, hydroxyl radicals, and intracellular reactive oxygen species (ROS) generated by hydrogen peroxide and ultraviolet B (UVB). Furthermore, CGA absorbed electromagnetic radiation in the UVB range (280?320 nm). UVB exposure resulted in damage to cellular DNA, as demonstrated in a comet assay; pre-treatment of cells with CGA prior to UVB irradiation prevented DNA damage and increased cell viability. Furthermore, CGA pre-treatment prevented or ameliorated apoptosis-related changes in UVB-exposed cells, including the formation of apoptotic bodies, disruption of mitochondrial membrane potential, and alterations in the levels of the apoptosis-related proteins Bcl-2, Bax, and caspase-3. Our findings suggest that CGA protects cells from oxidative stress induced by UVB radiation.
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KEYWORD
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Chlorogenic acid, Human keratinocyte, Ultraviolet B, Oxidative stress, Apoptosis
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